Ly-6G (Gr-1), clone: RB6-8C5, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5931-86
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (cat. 9668) be used when looking at Ly-6G specific targets.Applications Reported: The RB6-8C5 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen or formalin-fixed paraffin embedded tissue. It has also been reported in cell depletion. (Please use Functional Grade purified RB6-8C5, cat. 16-5931, in functional assays.).Applications Tested: The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The RB6-8C5 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Gr-1, Gr1, Ly-6G|
|Flow CytometryþFunctional AssayþImmunohistochemistryþImmunohistochemistry (Frozen)þImmunoprecipitationþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.