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Ly-6A/E (Sca-1) Monoclonal Antibody (D7), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

£126.00 - £286.00

Specifications

Antigen Ly-6A/E (Sca-1)
Clone D7
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15350810
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Affymetrix eBioscience
17-5981-81
50 μg
£126.00
50µg
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Estimated Shipment: 18-04-2024
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15360810
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Affymetrix eBioscience
17-5981-82
100 μg
£210.00
100µg
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Estimated Shipment: 18-04-2024
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15370810
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Affymetrix eBioscience
17-5981-83
200 μg
£286.00
200µg
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Description

Description

Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset. Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This D7 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Ly-6A/E (Sca-1) Monoclonal antibody specifically detects Ly-6A/E (Sca-1) in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

Ly-6A/E (Sca-1)
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1
Ly6a, Ly6e
Primary
4° C, store in dark, DO NOT FREEZE!
D7
Flow Cytometry
APC
Rat
Mouse
P05533, Q64253
110454, 17069
IgG2a κ
Affinity chromatography
Antibody
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