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Ly-49H Monoclonal Antibody (3D10), PE-Cyanine7, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 25-5886-82
Code : NEW
Additional Details : Weight : 0.09500kg
Description
Description: The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. It has been shown that there is preferential staining of Ly-49H with Ly-49D (cat. 12-5783). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly-49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells. Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3D10 antibody has been tested by fow cytometric analyis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Ly-49H Monoclonal antibody specifically detects Ly-49H in Mouse samples. It is validated for Flow Cytometry.Specifications
Ly-49H | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Q60682 | |
Klra8 | |
Affinity chromatography | |
RUO | |
16639 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
3D10 | |
PE-Cyanine7 | |
Klra8 | |
Cmv1; Cmv-1; cytomegalovirus resistance 1; killer cell lectin-like receptor 8; killer cell lectin-like receptor, subfamily A, member 8; Klra8; Ly49H; Ly-49h; Ly49-h; Ly49u<129>; Lymphocyte antigen 49h; T-cell surface glycoprotein Ly-49H | |
Mouse | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG1 κ |
For Research Use Only.