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Ly-49H, PE, clone: 3D10, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5886-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15507046

  • £49.99 / 25µg


Estimated Shipment date
from Supplier 29-05-2018
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Description

Description

Description: The monoclonal antibody 3D10 recognizes Ly49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins. It has been shown that there is preferential staining of Ly49H with Ly49D (cat 12-5783). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D10 antibody has been tested by flow cytometric analysis of C57Bl/6 splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Ly-49H
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q60682
Klra8
IgG1, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
16639
Flow Cytometry
3D10
PE
Klra8
Conjugated
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
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