Ly-49D Rat anti-Mouse, PerCP-eFluor 710, Clone: eBio4E5 (4E5), eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 46-5782-82

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15539276

Quantity Price
1 £ 195.53 / 100µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Concentration 0.2mg/mL
Host Species Rat
Conjugate PerCP-eFluor 710
Antigen Ly-49D
Species Reactivity Mouse
Primary or Secondary Primary
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Format Conjugated
Regulatory Status RUO
Isotype IgG2a
Monoclonal or Polyclonal Monoclonal
Applications Flow Cytometry
Quantity 100μg
Clone eBio4E5 (4E5)
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer

The monoclonal antibody eBio4E5 (4E5) reacts with Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R. eBio4E5 is reported to have functional (activating) activity.