Ly-49D, APC, clone: eBio4E5 (4E5), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 17-5782-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionCell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.