Ly-49C/I/F/H Monoclonal Antibody (14B11), Biotin, eBioscience™, Invitrogen™

Syrian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-5991-81

Product Code. 15239989

Description

Description: The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody reacts with 3 putative inhibitory members of the Ly-49 family, F, I, and C and one putative activating receptor, Ly-49H. 14B11 does not react with Ly-49A, B, D, or G. It is reported that 14B11 enhances the redirected lysis of FcR+ target cells by IL-2-activated NK cells. Applications Reported: The 14B11 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 14B11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

KLRA3 (also known as Ly49C) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA3 is thought to be an inhibitory receptor, recognizing peptide -receptive H-2Kb.

Specifications

• Antigen: Ly-49C/I/F/H
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q60653, Q64329, Q60682
• Gene Symbols: Klra3, Klra6, Klra8
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16634, 16637, 16639
• Content And Storage: 4° C, do not freeze
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 14B11
• Conjugate: Biotin
• Gene: Klra8
• Gene Alias: Killer cell lectin-like receptor 3, 5E6, Lymphocyte antigen 49c, Nk2.1, T-cell surface glycoprotein Ly-49C, Klra3, Ly-49c, Ly49C, Killer cell lectin-like receptor 6, Lymphocyte antigen 49f, Ly-49f, T-cell surface glycoprotein Ly-49F, Klra6, Ly-49f, Ly49-f, Ly49F
• Host Species: Syrian Hamster
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

For Research Use Only.