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Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, Low ROX
Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.
Brand: Thermo Scientific™ K0373
Code : 88
Additional Details : Weight : 0.09000kg
Includes:
- Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dy
Description
- Blue master mix and Yellow Sample Buffer for easy pipetting
- UDG in the master mix to prevent carry-over contamination
- Equal performance of blue and colorless master mixes
- Versions with premixed ROX or fluorescein for different real-time platforms
- Standard cycling protocols
- Excellent qPCR performance
- Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity: single copy detection
- Wide linear range: accurate quantification across 9 orders of magnitude
- Reproducibility and convenience: ready-to-use 2X master mix
Specifications
Detection Method: SYBR, PCR Method: qPCR | |
1250 Reactions | |
Gene expression analysis; siRNA validation; Genotyping; Pathogen detection | |
1250 | |
Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized. |
7500 System, QuantStudio™, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA™ 7 System | |
DNA, dsDNA | |
Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water | |
1250 x 20µL rxns |
For Research Use Only. Not for use in diagnostic procedures.