Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, Low ROX

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Brand:  Thermo Scientific™ K0373

Product Code. 13263179

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.

Includes:

• Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dy

Description

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance
• Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity: single copy detection
• Wide linear range: accurate quantification across 9 orders of magnitude
• Reproducibility and convenience: ready-to-use 2X master mix

Specifications

• Detection Method: Detection Method: SYBR, PCR Method: qPCR
• No. of Tests: 1250 Reactions
• For Use With (Application): Gene expression analysis; siRNA validation; Genotyping; Pathogen detection
• No. of Reactions: 1250
• Storage Requirements: Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.

• For Use With (Equipment): 7500 System, QuantStudio™, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA™ 7 System
• Sample Type: DNA, dsDNA
• Includes: Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
• Quantity: 1250 x 20µL rxns