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Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, Low ROX

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Brand:  Thermo Scientific™ K0371

UNSPSC : 41106300

Code : 88

Additional Details : Weight : 0.04000kg

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Product Code. 13243179

  • £154.00 / 250 reactions


Estimated Shipment
15-08-2017
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes:
  • Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dy

Specifications

Description

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
Description & Specifications

Specifications

Detection Method: SYBR, PCR Method: qPCR
250 Reactions
Gene expression analysis; siRNA validation; Genotyping; Pathogen detection
250
Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.
7500 System, QuantStudio™, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA™ 7 System
DNA, dsDNA
Hot Start Taq DNA Polymerase, UDG, and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; 40X Yellow Sample Buffer with a yellow dye; Nuclease-free water
250 x 20┬ÁL rxns