Invitrogen™ Levonorgestrel (LNG) Competitive ELISA Kit

Competitive ELISA

Brand:  Invitrogen™ EIALNGC

Product Code. 15924224

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.

Description

The Levonorgestrel (LNG) ELISA quantitates LNG in serum,plasma,urine,dried fecal extracts,saliva,milk,or cell culture medium.
Principle of the method
The Levonorgestrel (LNG) Competitive ELISA research-use-only kit is designed to quantitatively measure levonorgestrel present in serum,plasma,saliva,urine,dried fecal samples,milk,and tissue culture media samples. This kit measures total LNG in extracted serum,plasma,milk and fecal samples. A levonorgestrel stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A levonorgestrel-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to levonorgestrel to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound levonorgestrel-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Levonorgestrel (4-Estren-17alpha-Ethynyl-18-homo-17beta-ol-3-one (LNG) is a synthetic steroid analogue.

Specifications

• Assay Range: 6.25-200 pg/mL
• Description: Levonorgestrel (LNG) Competitive ELISA Kit
• Conjugate: HRP
• Product Type: ELISA Kit
• Sample Type: Fecal Extract, Milk, Plasma, Saliva, Serum, Supernatant, Urine, Water
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Intraassay CV: 9%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Fecal Extract,100 μL; Milk,100 μL; Plasma, 100 μL; Saliva,50 μL; Serum, 100 μL; Supernatant, 100 μL; Urine,25 μL; Water,100 μL
• Target: Chemical
• Total Assay Time: 1 hr. 30 min.

• Assay Sensitivity: 2.2 pg/mL
• Assay: Multiplex assay on the Luminex Platform
• Detection Method: Fluorescence
• Protein Family: Synthetic steroid hormone
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• Interassay CV: 9.1%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Reproductive Biology
• Storage Requirements: -20°C
• Test Time: 1 hr. 20 min.