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LAT, clone: LAT.10-17 (10-17), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9967-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15288287

  • £132.01 / 100µg


Estimated Shipment
26-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This LAT.10-17 (10-17) monoclonal antibody reacts with human Linker for Activation of T cells (LAT), which is essential for T cell receptor (TCR)-mediated signal transduction. Characterized as an integral transmembrane protein, this ∽37-kDa molecule does not possess a true extracellular domain. Upon TCR/CD3 engagement, LAT localizes to lipid rafts and is tyrosine phosphorylated by ZAP-70, leading to the recruitment and activation of other downstream signaling molecules such as Grb-2, PLCgamma, and PI3K. This adapter molecule has been shown to be required for T cell activation, as well as thymocyte and T regulatory cell development. In humans, LAT is expressed on peripheral blood lymphocytes, lymph nodes, and tonsil. Studies in mice have shown that LAT is expressed in thymocytes, T cells, mast cells, natural killer cells, megakaryocytes, platelets, and early B cells. This antibody does not crossreact with the mouse antigen.Applications Reported: This LAT.10-17 (10-17) antibody has been reported for use in immunoblotting (WB) and immunoprecipitation (IP).Applications Tested: LAT.10-17 (10-17) was tested by western blot and immunoprecipitation on lysates prepared from human peripheral blood cells, Jurkat cells, and C57BL/6 splenocytes. This antibody can be used at 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

LAT
LAT.10-17 (10-17)
Unconjugated
Purified
Murine
100μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Linker for activation of T-cells
IgG1
RUO
Primary
Human
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