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LAP (Latency Associated peptide), APC, clone: FNLAP, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-9829-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15518266

  • £211.52 / 100 tests


EU Stock 5
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Description

Description

Description: The FNLAP monoclonal antibody reacts with human latency associated peptide (LAP, pro-TGF beta 1, LAP/TGF beta 1). Many different cells produce TGF beta and it mediates effects on the proliferation, differentiation and function of many cell types. TGF beta is synthesized as a precursor that contains LAP at the N-terminus and mature TGF beta at the C-terminus. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells. It is believed that this surface-expressed LAP is due to the binding of LAP to GARP (LRRC32), which is a transmembrane protein that is also found at high levels on platelets and activated regulatory T cells.Applications Reported: This FNLAP antibody has been reported for use in flow cytometric analysis.Applications Tested: This FNLAP antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5μL (0.5μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

LAP (Latency Associated Peptide)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P01137
CED, DPD1, LAP, TGFB, TGFbeta
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
FNLAP
APC
TGFB1
Conjugated
TGFB1
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
7040
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