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Invitrogen™ LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit (Goat)

Brand:  Invitrogen™ PV4687

Code : 50

Additional Details : Weight : 0.09000kg

Product Code. 10276503

  • £1150.00 / Pack of 800


Estimated Shipment date
from Supplier 09-06-2020
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15136:

The LanthaScreen™ TR-FRET TR (thyroid receptor) alpha Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential TR alpha ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant TR alpha ligand-binding domain (TR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

To Assay:
When performing the LanthaScreen™ TR-FRET TR alpha coactivator assay, TR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the TR alpha-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, induce a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit contains TR alpha-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications

Specifications

LanthaScreen™ TR-FRET TR Alpha Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation
Pharma and biopharma, drug discovery and development, target and lead identification and validation, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor coregulator interaction
384-well plate
LanthaScreen™ TR-FRET TR Alpha Coactivator Assay Kit
800 x 20μL Assays
800 x 20μL Assays
Fluorescent
Microplate reader
TR alpha-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers
LanthaScreen™ TR-FRET TR Alpha Coactivator Assay Kit
For research use only
–68 to –85°C
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For Research Use Only. Not for use in diagnostic procedures.