Invitrogen™ LanthaScreen™ TR-FRET RXR alpha Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET RXR alpha Coactivator Assay Kit, goat

Brand:  Invitrogen™ PV4797

Product Code. 10636465

Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15138:

The LanthaScreen™ TR-FRET Retinoid X Receptor alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential RXR alpha ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti- GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RXR alpha ligand-binding domain (RXR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen™ TR-FRET Retinoid X Receptor alpha Coactivator Assay in agonist mode (to identify agonist compounds), RXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the TR-FRET ratio of 520/495 is calculated and can be used to determine the EC50 from a dose-response curve of the compound. Based on the biology of the RXR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see Figure 1).

Specifications

• Conjugate: Tb (Terbium)
• For Use With (Equipment): Microplate Reader
• Content And Storage: LanthaScreen™ TR-FRET RXR alpha Coactivator Assay Kits contain RXR alpha-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody and buffers. Store components as indicated in the assay protocol (-80°C, -20°C or 4°C).
• Readout: End Point
• Target Entry: RXRA, RXR alpha, NR2B1
• For Use With (Application): Co-Factor Interaction Assay, TR-FRET
• Product Type: TR-FRET RXR Alpha Coactivator Assay Kit

• Detection Method: Fluorescent
• Packaging: 384-well Plate
• Assay Entry: Biochemical coregulator interaction
• Shipping Condition: Dry Ice
• Product Line: LanthaScreen™
• Ligand: RXR alpha

For Research Use Only. Not for use in diagnostic procedures.