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Invitrogen™ LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit, rabbit
LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit (Rabbit)
Brand: Invitrogen™ A15128
Code : NEW
Additional Details : Weight : 0.09000kg
Description
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4655:The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR alpha ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.
In the LanthaScreen™ TR-FRET LXR alpha Coactivator Assay, LXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
Contents and Storage:
The LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit contains LXR alpha-LBD (GST) protein, fluorescently labeled TRAP220/DRIP-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications
Green | |
Fluorescent | |
Tb-anti-GST antibody (rabbit), LXR alpha-LBD, GST, fluorescein coactivator peptide, TR-FRET coregulator buffer H, 1M DTT | |
For research use only (RUO) | |
-80°C/-20°C/4°C | |
LanthaScreen™ TR-FRET LXR Alpha Coactivator Assay Kit |
LanthaScreen™ TR-FRET LXR Alpha Coactivator Assay Kit, 800 x 20μL Assays, Green, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification, Validation | |
Microplate reader | |
800 x 20 μL Assays | |
384-well Plate | |
Pharma and biopharma, drug discovery and development, target and lead identification and validation, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor coregulator interaction |
For Research Use Only. Not for use in diagnostic procedures.