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Invitrogen™ LanthaScreen™ TR-FRET ERR alpha Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET ERR alpha Coactivator Assay Kit, goat

Brand:  Invitrogen™ PV4663

Code : 50

Additional Details : Weight : 0.09000kg

Product Code. 10363283

  • £1156.00 / Pack of 800


Estimated Shipment date
from Supplier 18-06-2020
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15130:

The LanthaScreen™ ERR (estrogen related receptor) alpha TR-FRET Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential ERR alpha ligands as inverse agonists (sometimes referred to as antagonists) of coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ERR alpha ligand-binding domain (ERR alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.


In the LanthaScreen™ ERR alpha TR-FRET Coactivator Assay, ERR alpha-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the ERR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1).


Contents and Storage:


The LanthaScreen™ TR-FRET ERR alpha Coactivator Assay Kit contains ERR alpha-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications

Specifications

Biochemical coregulator interaction
2101
End Point
ESRRA, NR3B1, ERR1, ERR alpha
Co-Factor Interaction Assay
Fluorescent
384-well plate
Store in ultra-cold freezer (-68 to -85°C).
LanthaScreen®
Dry Ice
TR-FRET
Tb (Terbium)
Microplate Reader
Videos
Documents

For Research Use Only. Not for use in diagnostic procedures.