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Invitrogen™ LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, rabbit
LanthaScreen™ TR-FRET CAR Coactivator Assay Kit (Rabbit)
Brand: Invitrogen™ A15141
Code : NEW
Additional Details : Weight : 0.09000kg
Description
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4836:The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of liganddependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay:
When running the LanthaScreen™ TR-FRET CAR Coactivator Assay, CAR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and terbium anti-GST antibody. After an incubation period at room temperature, the 520 nm/ 495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the CAR-coactivator peptide interaction, this ligand’s EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and either recruit or displace coactivator peptide (Figures 1 and 2).
Specifications
Green | |
Fluorescent | |
Tb-anti-GST antibody (rabbit), CAR-LBD, GST, fluorescein coactivator peptide, TR-FRET coregulator buffer G, 1M DTT | |
For research use only (RUO) | |
-80°C/-20°C/4°C | |
LanthaScreen™ TR-FRET CAR Coactivator Assay Kit |
LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, 800 x 20μL Assays, Green, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation | |
Microplate reader | |
800 x 20 μL Assays | |
384-well Plate | |
Pharma and biopharma, drug discovery and development, target and lead identification and validation, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor coregulator interaction |
For Research Use Only. Not for use in diagnostic procedures.