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KLRG1, PE-Cyanine7, clone: 13F12F2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-9488-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15548666

  • £93.40 / 25 tests


EU Stock 5
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Disclaimers:

For Research Use Only.


Description

Description

Description: This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitor receptor of the C-type lectin superfamily that contains an ITIM domain. This inhibitory receptor is expressed on subsets of gamma-delta T cells, NK (CD56dim), CD8+ and CD4+ T Cells. KLRG1 is expressed primarily by cells with an effector/memory phenotype that are short-lived, but capable of immediate effector cell function. Cadherin-E, -N, and -R are ligands for KLRG1. Cadherin/KLRG1 interaction inhibits cytolytic activity and proliferation. The percentage of KLRG1 positive cells can vary considerably, depending on antigen experience.The clones 13F12F2 and 13A2 appear to recognize a similar epitope based on cross-blocking studies.Applications Reported: This 13F12F2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 13F12F2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

KLRG1
13F12F2
PE-Cyanine7
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal
Flow Cytometry
5μL (0.06μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
MAFA
IgG2a
RUO
Primary
Human
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