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KLRG1 Syrian Hamster anti-Mouse, PE-eFluor® 610, Clone: 2F1, eBioscience™

Syrian Hamster Monoclonal Antibody

£80.25 - £194.00

Specifications

Antigen KLRG1
Clone 2F1
Target Species Mouse
Conjugate PE-eFluor™ 610
Isotype IgG
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15590527 View Documents Affymetrix eBioscience
61-5893-80
25 μg £80.25
25µg View alternative products
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15500537 View Documents Affymetrix eBioscience
61-5893-82
100 μg £194.00
100µg View alternative products
Save Up to 
»
In stock, ready to ship: 1 Please to purchase this item. Need a web account? Register with us today!
Description

Description

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.
Specifications

Specifications

KLRG1
Mouse
IgG
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
2F1
PE-eFluor™ 610
Flow Cytometry
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.