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KLRG1, eFluor 450, clone: 2F1, eBioscience™
Syrian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 48-5893-82
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.Applications Reported: This 2F1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2F1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
KLRG1 | |
Monoclonal | |
0.2mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
O88713 | |
2F1, CLEC15A, MAFA, MAFA-2F1, MAFA-L, MAFA-LIKE, C-type lectin domain family 15 member A, C-type lectin domain family 15, member A, ITIM-containing receptor MAFA-L, MAFA-like receptor, killer cell lectin-like receptor subfamily G member 1, mast cell function-associated antigen (ITIM-containing) | |
Golden Syrian Hamster | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Murine |
Flow Cytometry | |
2F1 | |
eFluor 450 | |
Klrg1 | |
Conjugated | |
Klrg1 | |
IgG | |
100μg | |
Store at 2-8°C. Do not freeze. Light-sensitive material. | |
Primary | |
50928 |
For Research Use Only.