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KLRG1, Functional Grade, clone: 2F1, eBioscience™

Syrian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5893-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15218687

  • £80.93 / 100µg


Estimated Shipment
28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.Applications Reported: 2F1 has been reported for use in flow cytometric analysis and functional assays.Applications Tested: The 2F1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

KLRG1
2F1
Unconjugated
Purified
Golden Syrian Hamster
100μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
MAFA
IgG
RUO
Primary
Murine
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