KLRG1, Functional Grade, clone: 2F1, eBioscience™
Syrian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 16-5893-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.Applications Reported: 2F1 has been reported for use in flow cytometric analysis and functional assays.Applications Tested: The 2F1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|2F1, CLEC15A, MAFA, MAFA-2F1, MAFA-L, MAFA-LIKE, C-type lectin domain family 15 member A, C-type lectin domain family 15, member A, ITIM-containing receptor MAFA-L, MAFA-like receptor, killer cell lectin-like receptor subfamily G member 1, mast cell function-associated antigen (ITIM-containing)|
|Golden Syrian Hamster|
|Flow CytometryþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.