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Thermo Scientific™ Klenow Fragment

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Brand:  Thermo Scientific™ EP0052

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 10795071

  • £218.00 / 1500 units


EU Stock 12
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Specifications

Description

Highlights

  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
  • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications

  • DNA blunting by fill-in 3'-overhangs
  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • DNA sequencing by the Sanger method
  • Site-specific mutagenesis of DNA with synthetic oligonucleotides
  • Second strand synthesis of cDNA
Description & Specifications

Specifications

10U/┬ÁL
E.coli cells with a cloned fragment of the polA gene
1,500 U
metal chelators, PPi, Pi (at high concentrations) (8)
68kDa monomer