Invitrogen™ KIT [N822K] Recombinant Human Protein

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Recombinant human KIT N822K (544-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Product Code	Brand	Quantity
15448953	Invitrogen™ PV6310	10μg
15458953	Invitrogen™ PV6311	100μg
15478953	Invitrogen™ PV6312	1mg

Description

KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Specifications

• For Use With (Application): Enzyme and Protein Activity Assay, Industrial and Applied Science, Kinase Assay, Kinase Biology, Kinase Proteins and Expression Reagents, Pharma and Biopharma, Protein Assays and Analysis, Protein Biology, Target and Lead Identification and Validation
• Molecular Weight (g/mol): 75.3kDa
• Gene Alias: PBT, SCFR, CD117
• Species: Human
• Protein Tag: GST Tag

• Formulation: 50mM Tris-HCl pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
• Storage Requirements: Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Protein Length: aa 544-end
• Recombinant: Recombinant