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Ki-67, eFluor 570, clone: SolA15, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 41-5698-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15351070

  • £172.75 / 100µg


EU Stock 1
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Description

Description

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the Ki-67 labeling index refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.Applications Reported: This SolA15 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This SolA15 antibody has been tested immunocytochemistry on fixed and permeabilized C2C12 cells and can be used at less than or equal to 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

Ki-67
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
E9PVX6, P46013
antigen identified by monoclonal antibody Ki-67, antigen KI-67, KIA, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105, RP11-380J17.2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Rat
Immunohistochemistry (Frozen)
SolA15
eFluor 570
MKI67
Conjugated
Mki67
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
4288
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