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Ki-67, clone: SolA15, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5698-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15227437

  • £56.84 / 25µg


EU Stock 5
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Description

Description

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the Ki-67 labeling index refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.Applications Reported: This SolA15 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue sections and frozen (paraformaldehyde fixed) sections and immunocytochemistry (ICC).Applications Tested: This SolA15 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded mouse skin with high or low pH antigen retrieval and immunocytochemistry on fixed and permeabilized C2C12 cells at less than or equal to 5 ug/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Ki-67
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P46013, E9PVX6
antigen identified by monoclonal antibody Ki-67, antigen KI-67, KIA, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105, RP11-380J17.2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
CanineþCynomolgusþHumanþMurineþNon-human PrimateþRat
ImmunocytochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)
SolA15
Unconjugated
MKI67
Purified
MKI67
IgG2a, kappa
25μg
Store at 2-8°C.
Primary
4288
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