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Ki-67, Alexa Fluor 700, clone: SolA15, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-5698-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15520367

  • £205.37 / 100µg


EU Stock 2
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Disclaimers:

For Research Use Only.


Description

Description

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the Ki-67 labeling index refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This SolA15 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This antibody can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Ki-67
SolA15
Alexa Fluor 700
Conjugated
IgG2a
RUO
Primary
Rat
Flow Cytometry (Intracellular Staining)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Rat
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
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