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Ki-67 Mouse anti-Canine, Human, PE-Cyanine7, Clone: 20Raj1, eBioscience™

Mouse Monoclonal Antibody

£76.75 - £194.00

Specifications

Antigen Ki-67
Clone 20Raj1
Target Species Canine, Human
Conjugate PE-Cyanine7
Isotype IgG1, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15598556
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Affymetrix eBioscience
25-5699-41
25 Tests £76.75
25 tests View alternative products
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Estimated Shipment
25-11-2020
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15508566
View Documents
Affymetrix eBioscience
25-5699-42
100 Tests £194.00
100 tests View alternative products
Save Up to 
»
Estimated Shipment
25-11-2020
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Description

Description

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
Specifications

Specifications

Ki-67
Canine, Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
20Raj1
PE-Cyanine7
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.