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Ki-67, eFluor 660, clone: 20Raj1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-5699-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15559976

  • £75.82 / 25µg


Estimated Shipment date
from Supplier 30-07-2018
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Description

Description

Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells.This monoclonal antibody 20Raj1 recognizes canine Ki-67.Applications Reported: This 20Raj1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This 20Raj1 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized HeLa cells, immunohistochemistry on FFPE human skin using low pH antigen retrieval, and flow cytometry on stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. For immunocytochemistry immunohistochemistry, this can be used at less than or equal to 20 μg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.125 μg/test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Ki-67
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P46013
antigen identified by monoclonal antibody Ki-67, antigen KI-67, KIA, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105, RP11-380J17.2
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
CanineþHuman
Flow CytometryþImmunocytochemistryþImmunohistochemistryþMicroscopy
20Raj1
eFluor 660
MKI67
Conjugated
MKI67
IgG1, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
4288
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