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Ki-67, eFluor 615, clone: 20Raj1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 42-5699-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15301120

  • £178.71 / 100µg


Estimated Shipment
27-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells.This monoclonal antibody 20Raj1 recognizes canine Ki-67.Applications Reported: This 20Raj1 antibody has been reported for use in immunocytochemical and immunohistochemical staining of paraffin-embedded tissue (IHC-P).Applications Tested: This 20Raj1 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized HeLa cells, and immunohistochemistry on FFPE human skin using low pH antigen retrieval. For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Ki-67
20Raj1
eFluor 615
Conjugated
IgG1
RUO
Primary
Human
Immunohistochemistry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
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