missing translation for 'onlineSavingsMsg'
Learn More

Ki-67, Biotin, clone: 20Raj1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-5699-80

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15527346

  • £75.64 / 25µg


Estimated Shipment date
from Supplier 27-06-2018
Add to basket

Description

Description

Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells.This monoclonal antibody 20Raj1 recognizes canine Ki-67.Applications Reported: This 20Raj1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.Applications Tested: This 20Raj1 antibody has been tested by intracellular staining of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffers Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The 20Raj1 antibody has also been tested by immunocytochemistry of fixed and permeablilized cells and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Ki-67
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P46013
antigen identified by monoclonal antibody Ki-67, antigen KI-67, KIA, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105, RP11-380J17.2
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
CanineþHuman
Flow CytometryþImmunocytochemistryþImmunohistochemistryþMicroscopy
20Raj1
Biotin
MKI67
Conjugated
MKI67
IgG1, kappa
25μg
Store at 2-8°C. Do not freeze.
Primary
4288
Videos

For Research Use Only.