Jagged 2 Monoclonal Antibody (HMJ2-1), eFluor™ 660, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Product Code	Brand	Quantity
15519936	Affymetrix eBioscience 50-3392-82	100 μg
15509936	Affymetrix eBioscience 50-3392-80	25 μg

Description

Description: This HMJ2-1 monoclonal antibody reacts with mouse Jagged-2, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g., Notch 1-4), Jagged-2 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Jagged-2 is expressed by thymic lymphoid and stromal cells, as well as macrophages and dendritic cells in the spleen. Note: Staining of endogenous splenic dendritic (CD8+CD11c+) cells could only be consistently confirmed in cells isolated from C57BL/6, but not Balb/c, mice. Other strains have not been tested. The antiobdy has been reported to crossreact to rat and have blocking function. Applications Reported: This HMJ2-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HMJ2-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647.

Jagged 2 Monoclonal antibody specifically detects Jagged 2 in Mouse, Rat samples. It is validated for Flow Cytometry.

Specifications

• Antigen: Jagged 2
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene ID (Entrez): 16450, 29147
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: HMJ2-1
• Applications: Flow Cytometry
• Conjugate: eFluor 660
• Host Species: Armenian Hamster
• Target Species: Mouse, Rat
• Gene Symbols: Jag2
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: Jag2

For Research Use Only.