ISG15, clone: 3C2, eBioscience™
Armenian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5857-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: This 3C2 monoclonal antibody reacts with mouse interferon stimulated gene-15 (ISG-15), a 15kDa ubiquitin-like protein (UBL) and cytokine. ISG-15 is translated as a precursor that is subsequently activated by an E1 enzyme, conjugated by an E2 enzyme via the C-terminal LRLRGG motif and ligated by an E3 ligase. The activity of ISG-15 is dependent on its modification of target proteins at specific lysine residues. Several viruses utilize ISG-15 and ubiquitin deconjugating proteases, while influenza B protein NS1 has been shown to specifically bind ISG-15 thereby blocking its conjugation.In addition to its role as a protein modification, ISG-15 is secreted by monocytes and lymphocytes in response to IFNalpha, beta, or gamma, as well as influenza B virus. In this role as cytokine, it has been shown to induce secretion of IFNgamma and support NK cell proliferation and cytolytic activity.Applications Reported: This 3C2 antibody has been reported for use in immunoblotting (WB).Applications Tested: This 3C2 antibody has been tested by immunoblot of Raw 264.7 cell lysates at 2 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|G1P2, IFI15, UCRP|
|Store at 2-8°C.|
For Research Use Only.