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IRF8 Mouse anti-Human, Mouse, PerCP-eFluor™ 710, Clone: V3GYWCH, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9852-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15589436

  • £89.50 / 25µg

EU Stock 4

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Description

Description

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.
Specifications

Specifications

IRF8
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q02556, P23611
H-ICSBP, ICSBP, ICSBP1, IRF-8
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
Flow Cytometry
V3GYWCH
PerCP-eFluor™ 710
IRF8
Liquid
IRF8
IgG1, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
15900, 3394
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