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IRF7 Monoclonal Antibody (MNGPKL), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

£90.75 - £233.00

Specifications

Antigen IRF7
Clone MNGPKL
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15577026
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Affymetrix eBioscience
12-5829-80
25 μg
£90.75
25µg
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Estimated Shipment: 25-04-2024
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15587026
View Documents Promotion Details
Affymetrix eBioscience
12-5829-82
100 μg
£233.00
100µg
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Estimated Shipment: 04-04-2024
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Description

Description

Description: The monoclonal antibody MNGPKL reacts with mouse interferon regulatory factor 7 (IRF7), a 54 kDa transcription factor essential for induction of the antiviral innate immune response and IFN alpha production. B cells, plasmacytoid dendritic cells (pDC), and monocytes constitutively express IRF7 in the cytoplasm until activation by viral infection, double-stranded RNA or Toll-like receptor (TLR) signaling. CpG ODNs and single-stranded RNA, recognized by CD289 (TLR9) and TLR7, respectively, trigger IRF7 activation and upregulation. This activation promotes type I IFN response to disrupt pathogen and viral infection. These events cause IRF7 phosphorylation and translocation to the nucleus where it forms a homodimer to affect transcription. Applications Reported: This MNGPKL antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MNGPKL antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.
Specifications

Specifications

IRF7
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
IMD39; Interferon regulatory factor 7; interferon regulatory factor-7H; Irf7; IRF-7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
IRF7
Primary
4° C, store in dark, DO NOT FREEZE!
IRF7
MNGPKL
Flow Cytometry
PE
Rat
Mouse
P70434
54123
IgG2a κ
Affinity chromatography
Antibody
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