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IRF7, PE, clone: MNGPKL, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5829-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15587026

  • £180.65 / 100µg


EU Stock 5
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Description

Description

Description: The monoclonal antibody MNGPKL reacts with mouse interferon regulatory factor 7 (IRF7), a 54kDa transcription factor essential for induction of the antiviral innate immune response and IFN alpha production. B cells, plasmacytoid dendritic cells (pDC), and monocytes constitutively express IRF7 in the cytoplasm until activation by viral infection, double-stranded RNA or Toll-like receptor (TLR) signaling. CpG ODNs and single-stranded RNA, recognized by CD289 (TLR9) and TLR7, respectively, trigger IRF7 activation and upregulation. This activation promotes type I IFN response to disrupt pathogen and viral infection. These events cause IRF7 phosphorylation and translocation to the nucleus where it forms a homodimer to affect transcription.Applications Reported: This MNGPKL antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MNGPKL antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IRF7
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P70434
IRF-7H, IRF7A
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
MNGPKL
PE
Irf7
Conjugated
Irf7
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
54123
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