missing translation for 'onlineSavingsMsg'
Learn More

IRF4 Rat anti-Human, Mouse, PerCP-eFluor™ 710, Clone: 3E4, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9858-82

Code : NEW

Additional Details : Weight : 0.09500kg

Product Code. 15579446

  • £224.00 / 100µg

Estimated Shipment date
from Supplier 24-03-2020

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.
Specifications

Specifications

IRF4
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q64287, Q15306
LSIRF, MUM1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
Flow Cytometry
3E4
PerCP-eFluor™ 710
IRF4
Liquid
Irf4
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16364, 3662
Videos
Documents

For Research Use Only.