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IRF4, clone: 3E4, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9858-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15218197

  • £156.99 / 100µg


Estimated Shipment
26-07-2018
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Description

Description

Description: The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4). IRF4 is a 52kDa transcription factor with roles in B cell, T cell and macrophage function. In B cells, IRF4 is highly expressed in mature plasma cells and plays a crucial role in their differentiation. IRF4 has been shown to interact with PU.1 and control the transcription of many B cell-specific genes including Prdm1, which encodes Blimp1. In T cells, IRF4 has been implicated in regulatory T (Treg), T helper (Th)2, Th9 and Th17 cell development and function. This transcription factor is upregulated upon T cell activation and is expressed in mature T cells. Studies have shown that IRF4 directly induces Blimp1 expression in Tregs, leading to IL-10 expression. IRF4 has also been demonstrated to be involved in macrophage polarization and regulation. Lastly, in addition to its roles in normal immune function, IRF4 expression has been reported to be upregulated in many blood-related cancers.Applications Reported: This 3E4 antibody has been reported for use in western blotting.Applications Tested: This 3E4 antibody has been tested by western blot of reduced cell lysate prepared from splenocytes cultured for 3 days in the presence of plate-bound Anti-Mouse CD3 and soluble Anti-Mouse CD28. This antibody can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IRF4
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q64287, Q15306
LSIRF, MUM1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþMurine
Western Blot
3.00E+04
Unconjugated
IRF4
Purified
Irf4
IgG1, kappa
100μg
Store at 2-8°C.
Primary
3662
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