Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10 E. coli, without manual
Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning
Disclaimers: For Research Use Only. Not for use in diagnostic procedures.
Description and Specification
|Product Type||Kit, Cloning for Subcloning|
|Includes||Box 1: Topoisomerase I-activated pCR2.1-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid|
|For Use With (Application)||Cloning, PCR Cloning|