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Invitrogen™ TOPO™ TA Cloning™ Kit, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

£638.00 - £1012.00

Specifications

Bacterial or Yeast Strain DH5α
Cloning Method TOPO™-TA
Content And Storage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Line One Shot™
View More Specs

Includes: Topoisomerase I-activated pCR™2.1-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10624043
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Invitrogen™
K452001
25 Reactions
£638.00
Each
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Estimated Shipment: 08-04-2024
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10462862
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Invitrogen™
K452040
50 Reactions
£1012.00
Each
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Save Up to 
»
Estimated Shipment: 08-04-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
Description

Description

They use a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning

Specifications

Specifications

DH5α
Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
One Shot™
T7, SP6
TOPO™-TA
Kit
Cloning Kit
TOPO-TA Cloning Vectors
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.