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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

For fast, efficient cloning and subsequent in vitro transcription/translation

Brand:  Invitrogen™ K4600J10

234.01 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.25000kg

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Product Code. 15380204

  • £351.00 / 10 reactions
Estimated Shipment: 08-04-2024
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Description

Description

By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable method, taking only 5 minutes using a 3-step protocol, and yielding up to 95% recombinants. This kit includes the pCR™II-TOPO TA vector.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants
  • Includes OneShot™ TOP10 Competent cells for added convenience and highest cloning efficiencies

Applications:

Cloning, PCR Cloning

Specifications

Specifications

TOP10
Chemically Competent
Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
TOPO™
T7, SP6
TOPO-TA Vectors
Ampicillin (AmpR), Kanamycin (KanR)
TOPO™-TA
Kit
Cloning Kit
10 Reactions
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Special Offers

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For Research Use Only. Not for use in diagnostic procedures.