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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Brand:  Invitrogen™ K202040

266.01 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 50

Additional Details : Weight : 0.05000kg

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Product Code. 10594193

  • £399.00 / Each
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

  • Fast and convenient: 15-minute, room-temperature ligation
  • Efficient: blue/white screening and >80% clones with correct insert
  • Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
  • Hassle-free: eliminates any enzymatic modifications of the PCR product
  • Streamlined: does not require the use of PCR primers that contain restriction sites

  • Provides 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 promoter for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of insert
  • M13 forward and reverse primer sites for sequencing
  • How TA Cloning Works
  • Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
  • Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
  • Allows PCR inserts to ligate efficiently with vector

Cloning, PCR Cloning

Specifications

Specifications

Not Included
TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
PCR Cloning
TA Cloning™
T7
TA Cloning
Kit
40 Reactions
Cloning Kit
pCR2.1
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For Research Use Only. Not for use in diagnostic procedures.