T4 Polynucleotide Kinase

Catalyzes transfer of γ-phosphate of ATP to 5'hydroxyl terminus of DNA or RNA

Brand: Invitrogen

Manufacturer Part Number: 18004010


UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.02000kg

Product Code. 10790004

Quantity Price
1 £92.50 / Each
EU Stock 5
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Includes A vial of 5X forward reaction buffer [350mM Tris-HCl (pH 7.6), 50mM MgCl<sub>2</sub> , 500mM KCl, 5mM 2-mercaptoethanol] and a vial of 5X exchange reaction buffer [250mM imidazole-HCl (pH 6.4), 60mM MgCl<sub>2</sub> , 5mM 2-mercaptoethanol, 350μM ADP].
Quantity 200 units
Storage Requirements Store at -20°C

  • Source: Purified from E. coli expressing the T4 polynucleotide kinase gene in a λ vector
  • Performance and Quality Testing: Ribonuclease, endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease, and phosphatase assays; labeling efficiency tested
  • Unit Definition: One unit transfers 1nmol of phosphate of ATP to the 5' hydroxyl termini of micrococcal nuclease-treated DNA in 30 min. at 37°C
  • Unit Reaction Conditions: 70mM Tris-HCl (pH 7.6), 0.1M KCl, 10mM MgCl2 , 1mM 2-mercaptoethanol, 0.3mM [γ-32P]ATP, 0.38mg/mL micrococcal nuclease-treated DNA, and enzyme in 150μL for 30 min. at 37°C

5' end-labeling of DNA or RNA, Phosphorylation of synthetic linkers, Cloning, DNA & RNA Purification & Analysis, DNA Labeling, Nucleic Acid Labeling & Oligo Synthesis, Oligo Labeling, Restriction Enzyme Cloning