Store at -20°C, protected from light in a dessicator
SYBR Green I offers greater sensitivity for dsDNA, making it especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. With exceptionally low background fluorescence and spectral characteristics that closely match light sources and filter sets in existing instruments, SYBR Green I stain is ideal for use with laser scanners. Product use 1mL stains 100 minigels.
Sensitivity: at least four times greater than ethidium bromide for DNA in agarose gels
Ease of use: gels soaked in diluted stain can be visualized without desalting
Compatibility: with UV transilluminators, gel documentation systems, and laser scanners
Flexibility: for use with a broad range of applications, including DNA typing, PCR-based assays, DNA damage assays, analysis of complex samples, and real-time PCR detection
Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting