Invitrogen™ PureLink™ PCR Purification Kit

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.

Brand:  Invitrogen™ K310001

Product Code. 10751164

Description

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits. After PCR amplification, purification of the PCR amplicons is required for many common downstream applications, including enzyme digestion, ligation, sequencing, and labeling experiments, to prevent enzymes (polymerase, restriction enzyme) and reaction components (dNTPs, primers, buffers, salts) from carrying over, contaminating, and negatively influencing the downstream experiments.

Advantages of the PureLink PCR Purification Kit include:
• 4X binding capacity compared to Company Q; 40 μg for the PureLink kit versus 10 μg from other companies' kits
• No need for tedious sample pH adjustments necessary with other kits
• Convenient and rapid protocol, completed in less than 10 minutes
• Optional size-selection buffers included for selectively purifying 300–600 bp fragments or removing fragments <300 bp

The PureLink PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. Simply mix the PCR product with Binding Buffer and apply to the PureLink Spin Column. The dsDNA binds to the silica-based membrane in the column. Remove impurities by thorough washing with Wash Buffer. To purify the DNA, elute the dsDNA in low-salt Elution Buffer or water.The purified PCR product is suitable for automated fluorescent DNA sequencing, restriction enzyme digestion, and cloning.

Specifications

• Content And Storage: • 15 mL Binding Buffer (B2)
  • 23 mL Binding Buffer High-Cutoff (B3)
  • 16 mL Wash Buffer (W1)
  • 15 mL Elution Buffer (E1)
  • 50 PureLink™ PCR Spin Columns with Collection Tubes
  • 50 PureLink™ Elution Tubes (1.7 mL)
  
  Store at room temperature.
• High-throughput Compatibility: High-throughput Compatible
• Product Type: PCR Purification Kit
• Run Time: 10 min.
• Target: DNA from Enzymatic Reactions (e.g. PCR)
• Isolation Technology: Silica Spin Column

• Format: Kit
• Product Line: PureLink™
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• For Use With (Application): Next-Generation Sequencing (NGS), Cloning
• Sample Type: PCR Products

Safety and Handling

Product Identifier

• PURELINK PCR PURIFICATION KIT

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Guanidine hydrochloride

For Research Use Only. Not for use in diagnostic procedures.