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Invitrogen™ PureLink™ HiPure Plasmid Filter Maxiprep Kit

Purification in 90 minutes

Brand:  Invitrogen™ K210016

Code : 50

Additional Details : CAS Number : 9048-46-8 Weight : 0.12000kg Transport : UN number : 3266 Chem class : 8 Pack group : III

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Product Code. 10043432

  • £208.00 / 120mL

EU Stock 26

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

The PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of the highest purity from 25 to 100mL of overnight bacterial culture. The PureLink HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of bacterial lysate with anion-exchange resin purification of plasmid DNA, in single unit. The resulting plasmid DNA is of quality equivalent to that obtained by purification with two passes through cesium chloride gradients—the most rigorous method for DNA purification.

  • Purify high-quality DNA in Less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins
  • Additionally, elimination of phenol, chloroform, ethidium bromide, and cesium chloride from protocol minimizes exposure to and disposal of hazardous materials
PureLink HiPure Plasmid Filter Kits provide:
  • Simple protocols—bacterial lysate clarification without centrifugation
  • Fast results save time by purifying plasmid DNA in less than two hours
  • High yields up to 200μg for midipreps and up to 850μg for maxipreps
  • Reliable performance, high-quality plasmid DNA purified with these kits is suitable for wide variety of applications
How it works
  • Addition of a HiPure Filter Column to the PureLink HiPure Plasmid Kits provides rapid clearing of the bacterial lysate without the need for centrifugation
  • Lysate filtration cartridge is integrated into DNA-binding column for a combined step of lysate clarification and plasmid DNA binding directly to the anion-exchange resin
  • Single column wash removes impurities such as RNA, proteins, metabolites, and other low molecular weight molecules; the ultrapure plasmid DNA is then eluted with high-salt buffer
  • DNA is then desalted and concentrated with an isopropanol precipitation step, then collected by centrifugation

DNA & RNA Purification & Analysis, DNA Extraction, Maxiprep, Plasmid DNA Purification

Specifications

Specifications

Plasmid DNA
Plasmid DNA
Anion Exchange Resin with Lysate Clearing Filter
10 columns
The PureLink® HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt.
15 ml
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Transfection Grade
Room Temperature
PureLink™
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Bacterial Culture
10 Preps
1000 µg
15 ml
Not High-throughput Compatible (Manual)
> 200 µg (Large-Scale) Plasmid DNA
90 min
PureLink™
Videos
Documents

For Research Use Only. Not for use in diagnostic procedures.