Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit

Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Brand: Invitrogen™

Manufacturer Part Number: K480001

UNSPSC: 41106614

Code: 50

Additional Details:
Additional Details: Weight: 0.04000kg

Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10575383

Quantity Price
1 £ 482.0 / Each
EU Stock 8
Add to basket

For Research Use Only. All usage must comply with product instructions.

Description and Specification


Constitutive or Inducible System Constitutive
Delivery Type Transfection
Functions Available Constitutive Expression
Includes Box 1: 20μL pcDNA3.1/V5-His-TOPO (10ng/μL), 100μL 10X PCR Buffer, 10μL dNTP Mix (50mM); 50μL Salt Solution; 1mL Sterile Water; 10μL Control PCR Template (50ng/μL); 10μL Control PCR Primers (100ng/μL each); 20μL T7 Sequencing Primer (100ng/μL); 20μL BGH Reverse Sequencing Primer (100ng/μL); 10μL Expression Control Plasmid (500ng/μL); Box 2: 21 x 50μL TOP10 E. coli cells; 50μL pUC19 Control DNA (10pg/μL); 6mL S.O.C. Medium
Promoter CMV
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Quantity 20 reactions
Vector TOPO-TA Vectors, pcDNA
Storage Requirements Box 1: store at -20°C. Box 2: store at -80°C
Protein Tag His Tag (6X), V5 Epitope Tag