Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit

Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Overview
Brand: Invitrogen™

Manufacturer Part Number: K480040

UNSPSC: 41106614

Code: 50

Additional Details:
Additional Details: Weight: 0.14000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10584043

Quantity Price
1 £ 872.0 / Each
EU Stock 5
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Functions Available Constitutive Expression
Includes 2 x Box 1: 20μL pcDNA3.1/V5-His-TOPO (10ng/μL); 100μL 10X PCR Buffer; 10μL dNTP Mix (50mM); 50μL Salt Solution; 1mL Sterile Water; 10μL Control PCR Template (50ng/μL); 10μL Control PCR Primers (100ng/μL each); 20μL T7 Sequencing Primer (100ng/μL); 20μL BGH Reverse Sequencing Primer (100ng/μL); 10μL Expression Control Plasmid (500ng/μL). 2 x Box 2: 21 x 50μL TOP10 E. coli cells; 50μL pUC19 Control DNA (10pg/μL); 6mL S.O.C. Medium
Promoter CMV
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Quantity 40 reactions
Vector TOPO-TA Vectors, pcDNA
Storage Requirements Box 1: store at -20°C. Box 2: store at -80°C
Protein Tag His Tag (6X), V5 Epitope Tag