Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit

Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Brand:  Invitrogen™ K480040

Product Code. 10584043

Description

• No ligase, post-PCR procedures, or PCR primers containing specific sequences are required
• Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines
• pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody
• C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody
• Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants
• Vector features strong CMV promoter for high-level, constitutive expression
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Constitutive
• Promoter: CMV
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Quantity: 40 Reactions
• Product Line: TOPO™, pcDNA™

• Delivery Type: Transfection
• Product Type: TOPO TA Expression Kit
• Content And Storage: 2 × Box 1 (store at -20°C)
  • 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl)
  • 100 μl 10X PCR Buffer
  • 10 μl dNTP Mix (50 mM)
  • 50 μl Salt Solution
  • 1 ml Sterile Water
  • 10 μl Control PCR Template (50 ng/μl)
  • 10 μl Control PCR Primers (100 ng/μl each)
  • 20 μl T7 Sequencing Primer (100 ng/μl)
  • 20 μl BGH Reverse Sequencing Primer (100 ng/μl)
  • 10 μl Expression Control Plasmid (500 ng/μl)
  
  2 × Box 2 (store at -80°C)
  • 21 × 50 μl TOP10 E. coli cells
  • 50 μl pUC19 Control DNA (10 pg/μl)
  • 6 ml S.O.C. Medium
• Cloning Method: TOPO-TA
• Vector: TOPO-TA Vectors, pcDNA
• For Use With (Application): Constitutive Expression

For Research Use Only. Not for use in diagnostic procedures.