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Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

Brand:  Invitrogen™ K490040

Code : 50

Additional Details : Weight : 0.12000kg

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Product Code. 10472112

  • £848.00 / Each


Estimated Shipment
12-12-2017
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For Research Use Only. All usage must comply with product instructions.
 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Specifications

Constitutive
Constitutive Expression
CMV
40 reactions
Box 1: Store at -20°C); Box 2: Store at -80°C; 6mL S.O.C. Medium may be stored at 4°C or room temperature
Transfection
2 x Box 1: 20μL pcDNA3.1D/V5-His-TOPO (15-20ng/μL); 10μL dNTP Mix; 50μL Salt Solution; 1mL Sterile Water; 20μL T7 Sequencing Primer (0.1μg/μL); 20μL BGH Reverse Sequencing Primer (0.1μg/μL); 10μL Control PCR Template (0.1μg/μL); 10μL Control PCR Primers (0.1μg/μL each); 10μL Expression Plasmid (0.5μg/μL). 2 x Box 2: 6mL S.O.C. Medium; 21 x 50μL TOP10 Cells; 50μL pUC19 Control DNA
Geneticin™ (G-418)
Directional TOPO Vectors, pcDNA
His Tag (6X), V5 Epitope Tag
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