Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

Overview
Brand: Invitrogen™

Manufacturer Part Number: K490040

UNSPSC: 41106508

Code: 50

Additional Details:
Additional Details: Weight: 0.12000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10472112

Quantity Price
1 £ 848.0 / Each
EU Stock 3
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Constitutive or Inducible System Constitutive
Vector Directional TOPO Vectors, pcDNA
Storage Requirements Box 1: Store at -20°C); Box 2: Store at -80°C; 6mL S.O.C. Medium may be stored at 4°C or room temperature
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Delivery Type Transfection
Quantity 40 reactions
Protein Tag His Tag (6X), V5 Epitope Tag
Includes 2 x Box 1: 20μL pcDNA3.1D/V5-His-TOPO (15-20ng/μL); 10μL dNTP Mix; 50μL Salt Solution; 1mL Sterile Water; 20μL T7 Sequencing Primer (0.1μg/μL); 20μL BGH Reverse Sequencing Primer (0.1μg/μL); 10μL Control PCR Template (0.1μg/μL); 10μL Control PCR Primers (0.1μg/μL each); 10μL Expression Plasmid (0.5μg/μL). 2 x Box 2: 6mL S.O.C. Medium; 21 x 50μL TOP10 Cells; 50μL pUC19 Control DNA
Promoter CMV
Functions Available Constitutive Expression