Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

Overview
Brand: Invitrogen™

Manufacturer Part Number: K490040

PCDNA3.1 DIR TOPO EXPRESSION KIT 40 REACTIONS 00

UNSPSC: 41106508

Code: 50

Additional Details:
Additional Details: Weight: 0.12000kg



Product Code. 10472112

Quantity Price
1 £ 848.0 / Each
EU Stock 3
Add to basket

For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Functions Available Constitutive Expression
Includes 2 x Box 1: 20μL pcDNA3.1D/V5-His-TOPO (15-20ng/μL); 10μL dNTP Mix; 50μL Salt Solution; 1mL Sterile Water; 20μL T7 Sequencing Primer (0.1μg/μL); 20μL BGH Reverse Sequencing Primer (0.1μg/μL); 10μL Control PCR Template (0.1μg/μL); 10μL Control PCR Primers (0.1μg/μL each); 10μL Expression Plasmid (0.5μg/μL). 2 x Box 2: 6mL S.O.C. Medium; 21 x 50μL TOP10 Cells; 50μL pUC19 Control DNA
Promoter CMV
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Quantity 40 reactions
Vector Directional TOPO Vectors, pcDNA
Storage Requirements Box 1: Store at -20°C); Box 2: Store at -80°C; 6mL S.O.C. Medium may be stored at 4°C or room temperature
Protein Tag His Tag (6X), V5 Epitope Tag

  • Proofreading enzyme is used: fewer errors in cloned genes
  • Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
  • CMV promoter: high expression levels
  • C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
  • 6xHis tag for rapid protein purification on nickel-chelating resin

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis