Invitrogen™ NCode™ miRNA First-Strand cDNA Synthesis Kit

Part of a novel quantitative assay that provides flexibility, sensitivity, and specificity when quantitating miRNA expression

Overview
Brand: Invitrogen™

Manufacturer Part Number: MIRC10

NCODE MIRNA 1ST STRAND MOD

UNSPSC: 41106000

Code: NEW

Additional Details:
Additional Details: Weight: 0.02000kg



Product Code. 10461953

Quantity Price
1 £ 255.0 / Each
Estimated Shipment date
from Supplier 07-12-2016
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Description and Specification

Specification

Product Type Kit, miRNA First-Strand cDNA Synthesis
Includes The NCode First-Strand cDNA Synthesis Module provides enough reagents for 10 polyadenylation reactions and 20 reverse transcription reactions. The First-Strand cDNA Synthesis Module includes 5X miRNA Reaction Buffer, 25mM MnCl2, 10mM ATP, PolyA Polymerase, Annealing Buffer, SuperScript III Reverse Transcriptase/RNaseOUT Enzyme Mix, 2X First-Strand Reaction Buffer, Universal RT Primer (25μM), Universal qPCR Primer (10μM), and DEPC Water.
For Use With (Application) PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Real Time PCR-Based miRNA Analysis, Reverse Transcription, miRNA and Non-Coding RNA Analysis, miRNA Fluorescent Labeling for Microarrays
Quantity 10 reactions

The system combines a carefully optimized polyadenylation reaction with the market-leading reverse transcriptase, SuperScript™ III RT, in a universal first-strand cDNA synthesis reaction. The miRNA-specific amplification occurs during the subsequent PCR reaction, in which the sequence of the miRNA of interest is used as the target-specific PCR primer. Platinum™ SYBR™ GreenER™ qPCR SuperMix combines Platinum Taq DNA Polymerase with SYBR GreenER fluorescent dye, delivering excellent sensitivity in the quantitation of target sequences. The NCode First Strand cDNA Synthesis system is available separately as well as in a kit format with the Platinum SYBR; Green qPCR SuperMix or SYBR GreenER qPCR Reagent System.
The Platinum SYBR Green qPCR SuperMix combines Platinum Taq DNA Polymerase with SYBR Green fluorescent dye, delivering excellent sensitivity in the quantitation of target sequences.

SYBR GreenER qPCR Reagent System
The SYBR GreenER qPCR Reagent System uses a novel double-stranded DNA-binding dye with improved performance in qPCR. Compared to the original SYBR Green I, this dye produces a brighter signal and significantly reduces PCR inhibition while exhibiting nearly identical spectral characteristics. Use the SYBR GreenER qPCR Reagent System just like your SYBR Green qPCR kit, without changing instrument settings or filters to achieve superior performance from a broad range of targets.

  • A flexible assay for quantitating almost any miRNA as soon as the sequence is discovered
  • Sensitive detection over a broad range of input miRNA quantities, down to 100 copies
  • Single-nucleotide discrimination, providing specificity to profile closely related miRNA templates Platinum SYBR Green qPCR SuperMix
  • Optimal temperature: 50°C

PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Real Time PCR-Based miRNA Analysis, Reverse Transcription, miRNA and Non-Coding RNA Analysis, miRNA Fluorescent Labeling for Microarrays