Tfi DNA Polymerase

A new non-Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees

Brand: Invitrogen

Manufacturer Part Number: 30342011

100 RXN TFI DNA POLYMERASE 100RXN DDNSA # DAYS =210 CDNSA # days = 180 Special Storage Details =

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10640665

Quantity Price
1 £ 17.68 / 100 reactions
Estimated Shipment date
from Supplier 02-01-2017
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Exonuclease Activity 5' - 3'
Fidelity 1 X
GC-Rich PCR Performance Low
Includes Tfi DNA Polymerase is supplied with a vial of magnesium-free 5X Tfi PCR Buffer [200mM Hepes-KOH (pH 8.1), 75mM KCl, 75mM (NH4)2S04, 10% Glycerol and Proprietary stabilizers], and a vial of 50mM MgCl2.
No. of Reactions 100.0
Overhang 3'-A
Polymerase Tfi DNA Polymerase
Quantity 100 Reactions
Size (Final Product) 4 kb or less
Storage Requirements Store at -20°C. Guaranteed stable for 6 months when properly stored.

Like Taq, Tfi has 5' to 3' exonuclease and 5' to 3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness. Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4kb in length. Tfican be considered for use as a component in a diagnostic application if qualified by the end user.

  • Eliminate licensing fees and restrictions associated with Taq-based enzymes
  • Save money-Tfiis less expensive than TaqDNA Polymerase
  • Achieve yield, specificity, and fidelity comparable to Taq DNA polymerase
  • No need to reoptimize - swap out Taq for Tfi

Tfi is also available in special pack sizes and for OEM customers who may require PCR kit manufacturing flexibility.

PCR, PCR and Real-Time PCR, Routine PCR


Technical documents